BFE_Webpage_Project

Members

Junhua GUO

Shirley LI

Prof. Kayin LEUNG

Pinhe Liu from Prof. Harold Corke’s group

Yuying from Yigal’s lab

Tasks timeline

Kayin’s Lab –OK

Harold’s Lab

BEST lab –OK

BioFilm lab

Yigal’s lab

Remark

• The breakdown of every labs
• Pedro has a lab web page for the CrypEM and he can link it to Kayin’s six BFE labs later.
• The format can be under six categories: (a) Home to list out research areas, key words, research Qs, pictures, etc. (b) Research projects with photos. (c) Group members: such as PDF, Lab Managers, technicians, Graduate students and UGs. (d) Selective publications at GTIIT. (e) Openings. (f) Other activities. It is better to have pictures to show case the lab and videos are even better.

BEST

• BEST research areas 加图片或视频–不用了

• News & Events 不是很好看，图片不清晰 –放了有动画的组件–不管了

• Jiapeng Liu 没有图片

• 加了几个活动

Leung

• 图片裁剪 –还没剪
• vera的邮件和title –Shirley询问–要问–已问
• 两个phd的abstract –已完成
• home移过来 –已完成
• RI 漏的 –已完成
• 序号不知道要不要去掉 –待确认–两个的序号-要问–已问
• other activity 待确认
• suggest and review–已问–已回复
• group members 加人

Harold

• Home：内容不对，图片和内容都还是梁教授网页的 –OK
• Research Interests: pinhe的意思是做一个主页面把7个research interests的标题内容放上去，每一个research interest都做一个子页面，从主页面点击标题可以链接过去，可以参考https://sites.gtiit.edu.cn/cligroup/research-interests/
• Group Members: 这个页面看起来OK
• Publications: 这个页面看起来OK
• Main Facilities: 这个是要做一个主页面放4张图片，然后做4个子页面，放每个图片对应的facility，主页面的图片点击后可以链接到对应的子页面。–剩下要改的
• Invitation/Contact: 这个页面看起来OK

Oliver

• Lab layout – waiting for the videos and pictures
• Group members –no pictures

Yigal

• confirm the layout and the relationship of the pages

*Corresponding author

In natural environments, antibiotics are often present at sub-inhibitory concentrations. Antibiotic resistance genes (ARGs) may have initially evolved for environmental adaptation rather than resistance to clinically relevant antibiotics. The assembly of aresistome, which is the collection of all ARGs, within a developing microbial community remains poorly understood. Germfree models with low initial bacterial loads can unveil bacterial colonization and resistance development under an antibiotic pressure.Our studies aimed to address the following questions: (1) What are the key ARGs in soil and plant resistomes,and what are the core bacterial families, whether hosting ARGs or not? (2) How do initial bacterial loads and/or an antibiotic treatment influencethe evolution of soil’s and plant’s microbiomes and resistomes?(3) How does the plant resistome affect the antibiotic resistance of the foodborne pathogenSalmonella Typhimurium? (4) How does the resistome develop and look like in the pristine cave, which represents an environment with less human interventions in planetary history?
Soils with varying initial bacterial loads were initially exposed to a non-germfree environment and treated with different concentrations of tetracycline (TET). Both germfreesoil and TET treatments altered bacterial community and resistome profiles compared with untreated natural soil. Multidrug resistance (MDR)genes, especially multidrug efflux pumps, along with their primary host Burkholderiaceae, were dominant in the development of soil resistomes, rather than enhancing TET-related ARGs. Next, we inoculatedS.Typhimurium onto lettuce with germfree or natural leaves and grew them in germfree and natural soil,respectively, under various TET treatments. Only germfree soil affected the bacterial community and resistome profiles, with leaves not serving as a site forSalmonellaresistance development. MDR genes,particularly multidrug efflux pumps, and their primary host Burkholderiaceae, remained the key factors in resistome development in lettuce roots and soils. Lastly, we profiled bacterial communities and resistomes in 47publicly available datasets of 14 pristine cave environments. Microhabitats (sediments, microbial mats, water, biofilms, and minerals) in different environmental conditions led to distinct microbiome profiles. MDR genes, especially multidrug efflux pumps, and their bacterial hosts were highly prevalent and abundant. Variations in multidrug efflux pumps primarily accounted for significant differences in resistomes between microhabitats. This study represents a pioneering investigation into resistome assemblies within germfree soil and lettuce models, underscoring the significance of germfree models for resistome research. The findings emphasize the need to explore the inherent multidrug efflux pumps with their versatile capabilities, given that TET selections did not necessarily select for clinically relevant ARGs,and also may explain that the prevalence of mobile efflux pumps has been on the rise recently. Lastly, the analysis of resistomes in unspoiled cave environments reinforces the notion that resistomes originally emerged as a consequence of bacterial adaptation to their surroundings.